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{ version = 1.17; (* of floc.p 2022 Dec 16}
(* begin module describe.floc *)
(*
name
floc: fast find features of highest Ri in a given sequence range
synopsis
floc(features: in, flocp: in, bestfeatures: out,
lowerfeatures: out, output: out)
files
features: A list of locations of binding sites and other features.
bestfeatures: A list of the features from the original file which have the
highest Ri in a given range
flocp: parameters to control the program. The file must
contain the following parameters, one per line:
parameterversion: The version number of the program. This allows the
user to be warned if an old parameter file is used.
window: Features are discarded if the coordinate of another feature with
a higher Ri is less than the window distance away from the one being
looked at. Note that if window is 100, the region accepted will be
2*100+1 = 201 bases.
THIS FEATURE IS IGNORED in floc
asterisks: If character is 'A' then the filtered out features are shown
as asterisks in a lowerfeatures file.
NEW: and the same for bestfeatures.
maxsequences: The maximum number of sequences to process.
If maxsequences < 0, all sequences will be processed.
RangeFrom, RangeTo: the base range of the sequences to analyze.
lowerfeatures: A list of the features from the original file which
have the lower Ri values in a given range. These are the features
which have been 'discarded' by the program.
output: messages to the user
description
Floc is a fast local best program. The input is a list of features
found by the scan program in a Delila book containing nucleic acid
sequences. The set of features for each sequence in the book are
collected and the feature that has the highest Ri value is sent to
the bestfeatures file, while the remainder are sent to the
lowerfeatures file
The window parameter is IGNORED: everthing in a single sequence is
analyzed and the feature with the highest Ri is reported.
Scan often produces a features file with many sites picked up over
a small range. This makes the map very difficult to look at, so
this program attempts to filter the features and find the one with
the highest Ri over a small range and only keep that one.
The user should be aware that this program discards valuable
information in the form of possible binding sites. For this
reason, it is a good idea to not set the window at more than 1
helix turn (10.6 bp). Sites at this distance apart are probably
significant and should be kept in the features file.
examples
documentation
see also
localbest.p lister.p scan.p
author
Elaine Bucheimer wrote the original localbest.
Thomas Schneider completely rebuilt the program for speed in floc.
bugs
technical notes
*)
(* end module describe.floc *)
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